HSD17B1 ELISA Kit (Human) (OKCD00696)

Catalog No: OKCD00696 (Formerly GWB-KBBMB9)

Size:96WELLS

Human HSD17B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSD17B1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSD17B1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated HSD17B1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSD17B1
• Gene Full Name: hydroxysteroid 17-beta dehydrogenase 1
• Alias Symbols: 17-beta-HSD;17-beta-HSD 1;17-beta-hydroxysteroid dehydrogenase type 1;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;EDH17B2;EDHB17;estradiol 17-beta-dehydrogenase 1;HSD17;placental 17-beta-hydroxysteroid dehydrogenase;SDR28C1;short chain dehydrogenase/reductase family 28C member 1;short chain dehydrogenase/reductase family 28CE, member 1.
• NCBI Gene Id: 3292
• Protein Name: Estradiol 17-beta-dehydrogenase 1
• Description of Target: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
• Uniprot ID: P14061
• Protein Accession #: NP_000404
• Nucleotide Accession #: NM_000413.2
• Protein Size (# AA): 328