HRG ELISA Kit (Rat) (OKCD02545)

Catalog No: OKCD02545

Size:96WELLS

Rat HRG ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-99%	96-104%	94-101%	79-102%
  EDTA Plasma (n=5)	85-96%	95-103%	97-104%	93-101%
  Heparin Plasma (n=5)	81-91%	97-105%	89-103%	80-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Rich Glycoprotein (HRG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Rich Glycoprotein (HRG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Rich Glycoprotein (HRG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histidine Rich Glycoprotein (HRG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-96	85
  EDTA Plasma (n=5)	94-101	97
  Heparin Plasma(n=5)	78-94	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histidine Rich Glycoprotein (HRG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histidine Rich Glycoprotein (HRG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HRG Microplate: 96 Wells (12 x 8 Well strips)
  • HRG Lyophilized Standard: 2
  • 100X Biotinylated HRG Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.67 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histidine Rich Glycoprotein (HRG). No significant cross-reactivity or interference between Histidine Rich Glycoprotein (HRG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hrg|LOC681544
• Gene Full Name: histidine-rich glycoprotein|similar to histidine-rich glycoprotein
• Alias Symbols: histidine-proline-rich glycoprotein;histidine-rich glycoprotein;histidine-rich glycoprotein 1;histidine-rich glycoprotein 2;histidine-rich glycoprotein-like;HPRG;Hrg;HRG1;RNHRG2;similar to histidine-rich glycoprotein.
• NCBI Gene Id: 171016|681544
• Protein Name: Histidine-rich glycoprotein
• Description of Target: Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Inhibits rosette formation. Acts as an adapter protein and implicated in regulating many processes such as immune complex and pathogen clearance, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2 (By similarity).
• Uniprot ID: Q99PS8
• Protein Accession #: NP_001316829.1
• Nucleotide Accession #: NM_001329900.1
• Protein Size (# AA): 525