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Catalog No: OKEH04815
Size:96T
Price: $525.00
SKU
OKEH04815
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Hras ELISA Kit (Rat) (OKEH04815) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hras has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hras is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hras captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Hras Microplate96 Wells (12 x 8 Well Strips)
Hras Standard2
Sample Diluent1 x 20 mL
Hras Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Rat GTPase HRas
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHras
Gene Full NameHRas proto-oncogene, GTPase
Alias Symbolsc-H-ras;GTPase HRas;Harvey ras1 protein;Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog;Harvey rat sarcoma virus oncogene;H-Ras-1;HRAS1;p21ras;transforming protein p21.
NCBI Gene Id293621
Protein NameP20171
Description of TargetGTPase HRas
Uniprot IDhttps://www.uniprot.org/uniprot/P20171
Protein Accession #NP_001091711.1
Nucleotide Accession #NM_001098241.1
Protein Size (# AA)189
Molecular Weight21 kDa
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