Catalog No: OKEH04201
Size:96T
Price: $525.00
SKU
OKEH04201
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Hpgds ELISA Kit (Mouse) (OKEH04201) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Hpgds antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hpgds and incubated. The Hpgds found in the sample or standards competes with the biotinylated Hpgds for limited binding sites on the immobilized anti-mouse Hpgds antibody. Excess unbound biotinylated Hpgds and sample or standard Hpgds is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hpgds captured in the well and inversely proportional to the amount of Hpgds which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/ml | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 6.3% (n = 20) Mean Inter-assay CV%: <= 8.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
Sensitivity | 0.324 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Hematopoietic prostaglandin D synthase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | Hpgds |
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Gene Full Name | hematopoietic prostaglandin D synthase |
Alias Symbols | glutathione S-transferase;glutathione-dependent PGD synthase;glutathione-dependent PGD synthetase;glutathione-requiring prostaglandin D synthase;GST class-sigma;hematopoietic prostaglandin D synthase;H-PG;H-PGDS;prostaglandin D2 synthase 2, hematopoietic;prostaglandin-H2 D-isomerase;Ptgd;Ptgds2. |
NCBI Gene Id | 54486 |
Protein Name | Q9JHF7 |
Description of Target | Hematopoietic prostaglandin D synthase |
Uniprot ID | https://www.uniprot.org/uniprot/Q9JHF7 |
Protein Accession # | NP_062328.3 |
Nucleotide Accession # | NM_019455.4 |
Protein Size (# AA) | 199 |
Molecular Weight | 23 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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