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Catalog No: OKEH04201
Size:96T
Price: $525.00
SKU
OKEH04201
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Hpgds ELISA Kit (Mouse) (OKEH04201) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Hpgds antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hpgds and incubated. The Hpgds found in the sample or standards competes with the biotinylated Hpgds for limited binding sites on the immobilized anti-mouse Hpgds antibody. Excess unbound biotinylated Hpgds and sample or standard Hpgds is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hpgds captured in the well and inversely proportional to the amount of Hpgds which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Hpgds Microplate96 Wells (12 x 8 Well Strips)
Hpgds Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
100X Hpgds-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 10 mL
Biotin Complex Diluent1 x 120 uL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.324 ng/mL
SpecificityNatural and recombinant Mouse Hematopoietic prostaglandin D synthase
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolHpgds
Gene Full Namehematopoietic prostaglandin D synthase
Alias Symbolsglutathione S-transferase;glutathione-dependent PGD synthase;glutathione-dependent PGD synthetase;glutathione-requiring prostaglandin D synthase;GST class-sigma;hematopoietic prostaglandin D synthase;H-PG;H-PGDS;prostaglandin D2 synthase 2, hematopoietic;prostaglandin-H2 D-isomerase;Ptgd;Ptgds2.
NCBI Gene Id54486
Protein NameQ9JHF7
Description of TargetHematopoietic prostaglandin D synthase
Uniprot IDhttps://www.uniprot.org/uniprot/Q9JHF7
Protein Accession #NP_062328.3
Nucleotide Accession #NM_019455.4
Protein Size (# AA)199
Molecular Weight23 kDa
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