HOMER1 Antibody - N-terminal region (OAAB04284)

Catalog No: OAAB04284

Size:400ul

Western blot analysis of HOMER1 antibody (N-term) in HepG2 cell lysates (35ug/lane). HOMER1 (arrow) was detected using the purified Pab.

HOMER1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

Formalin-fixed and paraffin-embedded human hepatocarcinoma with HOMER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HOMER1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC
• Additional Information: Function: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
  Cellular Location: Cytoplasm (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HOMER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids from the N-terminal region of human HOMER1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HOMER1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: HOMER1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: HOMER1
• Alias Symbols: HOMER, SYN47, Ves-1, HOMER1A, HOMER1B, HOMER1C
• NCBI Gene Id: 9456
• Protein Name: Homer protein homolog 1
• Uniprot ID: Q86YM7
• Protein Accession #: NP_004263.1
• Molecular Weight: 40277 Da