Catalog No: OKDD00602 (Formerly GWB-KBBCC9)
Size:96 Wells
Price: $775.00
SKU
OKDD00602
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Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology HO1 ELISA Kit (Rat) (OKDD00602) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for HO1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HO1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-HO1 Microplate96 Wells (12 x 8 Well Strips)
HO1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X HO1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates.
Sensitivity<0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of HO1. No significant cross-reactivity or interference between HO1 and analogues was observed.
Publications

Amelioration of Cisplatin-Induced Ototoxicity in Rats by L-arginine: The Role of Nitric Oxide, Transforming Growth Factor Beta 1 and Nrf2/HO-1 Pathway. Asian Pac J Cancer Prev. 21, 2155-2162 (2020). 32711445

Effect of fish oil and telmisartan on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: The role of oxidative stress, transforming growth factor beta-1, and nuclear factor kappa B. Food Sci Nutr. 8, 5149-5159 (2020). 32994975

Assay InfoQuantitative Sandwich ELISA
Description
Gene SymbolHmox1
Gene Full Nameheme oxygenase 1
Alias Symbolsheme oxygenase (decycling) 1;heme oxygenase (decyclizing) 1;heme oxygenase 1;Heox;HEOXG;Hmox;Ho1;Ho-1;hsp32.
NCBI Gene Id24451
Protein NameHeme oxygenase 1
Description of TargetHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Uniprot IDP06762
Protein Accession #NP_036712.1
Nucleotide Accession #NM_012580.2
Protein Size (# AA)289
Molecular Weight33 kDa
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