HMOX1 ELISA Kit (Rat) (OKCD00246)

Catalog No: OKCD00246 (Formerly GWB-KBBPG1)

Size:96WELLS

Rat Hmox1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-90%	87-96%	79-91%	96-104%
  EDTA plasma(n=5)	82-95%	83-98%	96-103%	78-103%
  heparin plasma(n=5)	95-105%	90-97%	80-91%	88-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 1 (HO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 1 (HO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 1 (HO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heme Oxygenase 1 (HO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-102	91
  EDTA plasma(n=5)	79-95	92
  heparin plasma(n=5)	82-92	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Hmox1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Hmox1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Hmox1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1, Decycling (HO1). No significant cross-reactivity or interference between Heme Oxygenase 1, Decycling (HO1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Hmox1
• Gene Full Name: heme oxygenase 1
• Alias Symbols: heme oxygenase (decycling) 1;heme oxygenase (decyclizing) 1;heme oxygenase 1;Heox;HEOXG;Hmox;Ho1;Ho-1;hsp32.
• NCBI Gene Id: 24451
• Protein Name: Heme oxygenase 1
• Description of Target: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
• Uniprot ID: P06762
• Protein Accession #: NP_036712
• Nucleotide Accession #: NM_012580.2
• Protein Size (# AA): 289