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100 ul
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HMOX1 Antibody - N-terminal region (ARP45222_P050)

Description of Target:
HMOX1 belongs to the heme oxygenase family. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2.
Gene Symbol:
HMOX1
Official Gene Full Name:
Heme oxygenase (decycling) 1
NCBI Gene Id:
3162
Alias Symbols:
HO-1; HSP32; bK286B10
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HMOX1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HMOX1.
Swissprot Id:
P09601
Protein Accession #:
NP_002124
Nucleotide Accession #:
NM_002133
Host:
Rabbit
Clonality:
Polyclonal
Protein Name:
Heme oxygenase 1
Protein Size (# AA):
288
Molecular Weight:
33kDa
Application:
WB
Protein Interactions:
CCDC155; UBC; TUT1; CPSF1; ASL; POT1; TERF1; ELAVL1; HMX1; BLVRB; AKT1; NOS1; HMOX1; POR; UFD1L; PSMD2;
Immunogen:
The immunogen is a synthetic peptide directed towards the N-terminal region of Human HMOX1
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Purification:
Affinity Purified
Complete computational species homology data:
Anti-HMOX1 (ARP45222_P050)
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
Species Reactivity:
Cow; Dog; Goat; Guinea Pig; Human; Mouse; Pig; Rabbit; Rat
Datasheets / Downloads:
Printable datasheet for anti-HMOX1 (ARP45222_P050) antibody
Peptide Sequence:
Synthetic peptide located within the following region: ERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVM
Blocking Peptide:
For anti-HMOX1 (ARP45222_P050) antibody is Catalog # AAP45222
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Publications:

Kawahara, G. et al. Dystrophic muscle improvement in zebrafish via increased heme oxygenase signaling. Hum. Mol. Genet. 23, 1869-78 (2014). WB, Human, Dog, Goat, Rabbit, Mouse, Pig, Rat, Bovine, Guinea pig 24234649

Product Protocols: HMOX1 antibody tested with Human Hepg2 Cells (ARP45222_P050)

Aviva Systems Biology is the original manufacturer of this HMOX1 antibody (ARP45222_P050)

Click here to view the HMOX1 antibody Western Blot Protocol

Product Datasheet Link: HMOX1 antibody (ARP45222_P050)

WB Suggested Anti-HMOX1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2

Western Blot image:


Description of Target: HMOX1 belongs to the heme oxygenase family. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2.Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s HMOX1 antibody (ARP45222_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: HMOX1 antibody - N-terminal region (ARP45222_P050) in Human and Mouse tonsil using Western Blot

Product Page Link: HMOX1 antibody - N-terminal region (ARP45222_P050)

Data Provided by Dr. Frank Wagener of Radboud University, Netherlands

Tonsil Extract:

Took right side tonsil (19-04-2011 Corien) from -80 dC vial storage system. Cut off a piece of tonsil of about 3x3x3 mm and added 200ul Odyssey Lysis buffer (0.5% Triton X-100 + 1mM EDTA + 1mM PMSF + Protease Inhibitor Cocktail). Homogenized tonsil using an Eppendorf Micropestle and incubated on ice for about 30 minutes (vortexing every 10 minutes). Centrifuged full speed at 4 dC for 2 minutes and took supernatant. Measured protein concentration using the Nanodrop (BSA method) -->18.7mg/ml. Added to 300ul supernatant 100ul 4 x sample buffer and heated at 95 dC for 5 minutes -->14ug/ul. Stored tonsil extract in -80 dC vial storage system (vial drawer 4 box 1).

Western Blot

10% SDS-PAGE

HMOX-1 (Aviva): rabbit --> block 5% ELK (1h RT), 1st ab 1:3000 2.5% ELK + 0.1% Tween (O/N 4C), 2nd ab (680) 1:10000 2.5% ELK +0.1% Tween + 0.01% SDS (45 min RT).

Anti-Beta Actin (Sigma):, mouse --> block 5% ELK (1h RT) 1st ab 1:100000 2.5% ELK + 0.1% Tween (O/N 4C), 2nd ab (800) 1:10000 2.5% ELK + 0.1% Tween + 0.01% SDS (45min RT).

Used a 1:3000 dilution because only very little antibody was left (recommended dilution is 1:1000).

 
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