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Catalog No: OKCD02544
Size:96WELLS
Price: $725.00
SKU
OKCD02544
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Datasheets/ManualsPrintable datasheet for HMGCR ELISA Kit (Rat) (OKCD02544)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HMGCR Microplate96 Wells (12 x 8 Well strips)
HMGCR Lyophilized Standard2
100X Biotinylated HMGCR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.126 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). No significant cross-reactivity or interference between 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolHmgcr
Gene Full Name3-hydroxy-3-methylglutaryl-CoA reductase
Alias Symbols3H3M;3-hydroxy-3-methylglutaryl-coenzyme A reductase;HMG-CoA reductase.
NCBI Gene Id25675
Protein Name3-hydroxy-3-methylglutaryl-coenzyme A reductase
Description of TargetTransmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Uniprot IDP51639
Protein Accession #NP_037266.2
Nucleotide Accession #NM_013134.2
Protein Size (# AA)887
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