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Catalog No: OKEH00341
Size:96T
Price: $525.00
SKU
OKEH00341
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HMGB3 ELISA Kit (Human) : 96 Wells (OKEH00341)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HMGB3 ELISA Kit (Human) (OKEH00341) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMGB3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for HMGB3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMGB3 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery<= 3.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
HMGB3 Microplate96 Wells (12 x 8 Well strips)
HMGB3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HMGB3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human High mobility group protein B3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHMGB3
Gene Full Namehigh mobility group box 3
Alias Symbolshigh mobility group protein 2a;High mobility group protein 4;high mobility group protein B3;high-mobility group (nonhistone chromosomal) protein 4;HMG-2a;HMG2A;HMG4;HMG-4.
NCBI Gene Id3149
Protein NameO15347
Description of TargetHigh mobility group protein B3
Uniprot IDhttps://www.uniprot.org/uniprot/O15347
Protein Accession #NP_001288157.1
Nucleotide Accession #NM_001301228.1
Protein Size (# AA)200
Molecular Weight23 kDa
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