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| Datasheets/Manuals | Printable datasheet for HMGA1 Antibody (OAEB00890) |
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| Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | IHC |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | HMGA1 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 91%; Dog: 100%; Horse: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100% |
| Peptide Sequence | C-LASKQEKDGTEK |
| Concentration | 0.5 mg/ml |
| Preservative | 0.02% sodium azide |
| Specificity | This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b ((also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). The 50kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded human placenta shows strong nuclear staining. Recommended concentration: 4-6 ug/ml. |
| Reference | * Chiappetta G, Botti G, Monaco M, Pasquinelli R, Pentimalli F, Di Bonito M, D'Aiuto G, Fedele M, Iuliano R, Palmieri EA, Pierantoni GM, Giancotti V, Fusco A.; HMGA1 protein overexpression in human breast carcinomas: correlation with ErbB2 expression.; Clin Cancer Res. 2004 Nov 15;10(22):7637-44; Pubmed 15569996 |
| Gene Symbol | HMGA1 |
|---|---|
| Gene Full Name | high mobility group AT-hook 1 |
| Alias Symbols | high mobility group protein A1;high mobility group protein HMG-I/HMG-Y;high mobility group protein R;HMGA1A;HMGIY;HMG-R;nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y. |
| NCBI Gene Id | 3159 |
| Protein Name | High mobility group protein HMG-I/HMG-Y |
| Uniprot ID | P17096 |
| Protein Accession # | NP_665906.1 |
| Molecular Weight | 11.7; 10.7 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HMGA1 Antibody (OAEB00890)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "HMGA1 Antibody (OAEB00890)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HMGA1 Antibody (OAEB00890)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HMGA1 Antibody (OAEB00890)"?
This target may also be called "high mobility group protein A1;high mobility group protein HMG-I/HMG-Y;high mobility group protein R;HMGA1A;HMGIY;HMG-R;nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y." in publications.
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What is the shipping cost for "HMGA1 Antibody (OAEB00890)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HMGA1 Antibody (OAEB00890)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HMGA1 Antibody (OAEB00890)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "11.7; 10.7".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HMGA1 Antibody (OAEB00890)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HMGA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HMGA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HMGA1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HMGA1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HMGA1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HMGA1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
