HMGA1 Antibody - C-terminal region (OAAB05695)

Catalog No: OAAB05695

Size:400ul

HMGA1 Antibody (C-term) (Cat. #OAAB05695) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).

Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term)(Cat#OAAB05695).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).HMGA1 immunoreactivity is localized to Nucleus significantly.

HMGA1 Antibody (C-term) (Cat. #OAAB05695) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IF
  Function: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  Cellular Location: Nucleus. Chromosome.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Hamster; Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HMGA1 is supported by BioGPS gene expression data to be expressed in MDA-MB231

• Intended Use: HMGA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: HMGA1
• Alias Symbols: HMG-R, HMGIY, HMGA1A
• NCBI Gene Id: 3159
• Protein Name: High mobility group protein HMG-I/HMG-Y
• Description of Target: HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
• Uniprot ID: P17096
• Protein Accession #: NP_002122.1
• Molecular Weight: 11676 Da