HLA-G ELISA Kit (Human) : 96 Wells (OKEH00979)

Catalog No: OKEH00979 (Formerly GWB-D9XIJT)

Size:96T

Human HLA-G ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HLA-G ELISA Kit (Human) (OKEH00979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-G has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HLA-G is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HLA-G captured in well.
  
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • HLA-G Microplate: 96 Wells (12 x 8 Well strips)
  • HLA-G Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated HLA-G Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.11 ng/mL
• Specificity: Natural and recombinant Human HLA class I histocompatibility antigen, alpha chain G
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HLA-G
• Gene Full Name: major histocompatibility complex, class I, G
• Alias Symbols: b2 microglobulin;HLA class I histocompatibility antigen, alpha chain G;HLA G antigen;HLA-G histocompatibility antigen, class I, G;MHC class I antigen G;MHC class Ib antigen;MHC-G;mutant MHC class I antigen;mutant MHC class Ib antigen.
• NCBI Gene Id: 3135
• Protein Name: P17693
• Description of Target: HLA class I histocompatibility antigen, alpha chain G
• Uniprot ID: https://www.uniprot.org/uniprot/P17693
• Protein Accession #: NP_002118.1
• Nucleotide Accession #: NM_002127.5
• Protein Size (# AA): 338
• Molecular Weight: 38 kDa