Catalog No: OKEH01324
Size:96T
Price: $600.00
SKU
OKEH01324
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HLA-B27 ELISA Kit (Human) : 96 Wells (OKEH01324)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HLA-B27 ELISA Kit (Human) (OKEH01324) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-B27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HLA-B27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HLA-B27 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
HLA-B27 Microplate96 Wells (12 x 8 Well strips)
HLA-B27 Lyophilized Standard2
100X Biotinylated HLA-B27 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human HLA class I histocompatibility antigen, B-27 alpha chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHLA-B
Gene Full Namemajor histocompatibility complex, class I, B
Alias SymbolsAS;B-4901;HLA class I antigen HLA-B;HLA class I histocompatibility antigen, B alpha chain;HLAB;leukocyte antigen class I-B;major histocompatibility complex, class I, B;MHC class 1 antigen;MHC class I antigen B*27;MHC class I antigen HLA-B alpha chain;MHC class I antigen HLA-B heavy chain;MHC class I antigen SHCHA;MHC class I molecule;MHC HLA-B cell surface glycoprotein;MHC HLA-B transmembrane glycoprotein.
NCBI Gene Id3106
Protein NameP03989
Description of TargetHLA class I histocompatibility antigen, B alpha chain, HLA class I histocompatibility antigen, B-27 alpha chain
Uniprot IDhttps://www.uniprot.org/uniprot/P03989
Protein Accession #NP_005505.2
Nucleotide Accession #NM_005514.7
Protein Size (# AA)362
Molecular Weight40 kDa
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