HIST2H3A ELISA Kit (Human) (OKCD01095)

Catalog No: OKCD01095 (Formerly GWB-KBBBH5)

Size:96WELLS

Human HIST2H3A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	80-105%	96-105%	78-98%	82-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 2, H3a (HIST2H3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 2, H3a (HIST2H3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 2, H3a (HIST2H3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 2, H3a (HIST2H3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	85-96	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 2, H3a (HIST2H3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 2, H3a (HIST2H3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HIST2H3A Microplate: 96 Wells (12 x 8 Well Strips)
  • HIST2H3A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated HIST2H3A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Cluster 2, H3a (HIST2H3A). No significant cross-reactivity or interference between Histone Cluster 2, H3a (HIST2H3A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: H3C13|H3C14|H3C15
• Gene Full Name: H3 clustered histone 13|H3 clustered histone 14|H3 clustered histone 15
• Alias Symbols: H3;H3 histone family, member M;H3 histone, family 2;H3.2;H3/M;H3/n;H3/o;H3C13;H3C14;H3C15;H3-clustered histone 13;H3-clustered histone 14;H3-clustered histone 15;H3F2;H3FM;H3FN;HIST2H3A;HIST2H3C;HIST2H3D;histone 2, H3a;histone 2, H3c;histone 2, H3d;histone cluster 2 H3 family member a;histone cluster 2 H3 family member c;histone cluster 2 H3 family member d;histone cluster 2, H3a;histone cluster 2, H3c;histone cluster 2, H3d;histone H3.2;histone H3/m;histone H3/o.
• NCBI Gene Id: 126961|333932|653604
• Protein Name: Histone H3.2
• Description of Target: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Uniprot ID: Q71DI3
• Protein Accession #: NP_001005464
• Nucleotide Accession #: NM_001005464.2
• Protein Size (# AA): 136