Catalog No: OKCD01087 (Formerly GWB-KBBGP9)
Size:96WELLS
Price: $750.00
SKU
OKCD01087
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for HIST1H2AB ELISA Kit (Human) (OKCD01087) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H2ab (HIST1H2AB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 1, H2ab (HIST1H2AB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 1, H2ab (HIST1H2AB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 1, H2ab (HIST1H2AB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 1, H2ab (HIST1H2AB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 1, H2ab (HIST1H2AB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.107 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Histone Cluster 1, H2ab (HIST1H2AB). No significant cross-reactivity or interference between Histone Cluster 1, H2ab (HIST1H2AB) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | H2AC4|H2AC8 |
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Gene Full Name | H2A clustered histone 4|H2A clustered histone 8 |
Alias Symbols | H2A histone family, member A;H2A histone family, member M;H2A.1;H2A.2;H2A/a;H2A/m;H2AC4;H2AC8;H2AFA;H2AFM;HIST1H2AB;HIST1H2AE;histone 1, H2ab;histone 1, H2ae;histone cluster 1 H2A family member b;histone cluster 1 H2A family member e;histone cluster 1, H2ab;histone cluster 1, H2ae;histone H2A type 1-B/E;Histone H2A.2;histone H2A/a;histone H2A/m;histone H2AE. |
NCBI Gene Id | 3012|8335 |
Protein Name | Histone H2A type 1-B/E |
Description of Target | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Uniprot ID | P04908 |
Protein Accession # | NP_066390 |
Nucleotide Accession # | NM_021052.2 |
Protein Size (# AA) | 130 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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