Catalog No: OKCD01087 (Formerly GWB-KBBGP9)
Size:96WELLS
Price: $750.00
SKU
OKCD01087
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Datasheets/ManualsPrintable datasheet for HIST1H2AB ELISA Kit (Human) (OKCD01087)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
85-93%86-94%90-98%79-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H2ab (HIST1H2AB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 1, H2ab (HIST1H2AB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 1, H2ab (HIST1H2AB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 1, H2ab (HIST1H2AB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
85-10392
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 1, H2ab (HIST1H2AB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 1, H2ab (HIST1H2AB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HIST1H2AB Microplate96 Wells (12 x 8 Well Strips)
HIST1H2AB Lyophilized Standard2 x 20 ng
100X Biotinylated HIST1H2AB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.107 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Histone Cluster 1, H2ab (HIST1H2AB). No significant cross-reactivity or interference between Histone Cluster 1, H2ab (HIST1H2AB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolH2AC4|H2AC8
Gene Full NameH2A clustered histone 4|H2A clustered histone 8
Alias SymbolsH2A histone family, member A;H2A histone family, member M;H2A.1;H2A.2;H2A/a;H2A/m;H2AC4;H2AC8;H2AFA;H2AFM;HIST1H2AB;HIST1H2AE;histone 1, H2ab;histone 1, H2ae;histone cluster 1 H2A family member b;histone cluster 1 H2A family member e;histone cluster 1, H2ab;histone cluster 1, H2ae;histone H2A type 1-B/E;Histone H2A.2;histone H2A/a;histone H2A/m;histone H2AE.
NCBI Gene Id3012|8335
Protein NameHistone H2A type 1-B/E
Description of TargetCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Uniprot IDP04908
Protein Accession #NP_066390
Nucleotide Accession #NM_021052.2
Protein Size (# AA)130
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