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Catalog No: OAEA00010
Size:50ug
Price: $150.00
SKU
OAEA00010
Availability: Domestic: within 5-7 days | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for OAEA00010
Product Info
Product FormatLiquid 10mM PBS (pH 7.2) with 0.05% sodium azide
ClonalityMonoclonal
CloneHIS.H8
IsotypeIgG2b
HostMouse
ApplicationIF, WB, DB, IP, IS, ELISA
::Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins
Reconstitution and StorageCentrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks, and at -20°C for 2 years or more.
ImmunogenHHHHHH (6x His) synthetic peptide conjugated to KLH
PurificationProtein A affinity chromatography from mouse ascites fluid
Concentration1 mg/ml
SpecificityRecognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Application InfoWB (ECL): 1:1000~3000 (incubate for one hour at room temperature)
IP: 1:50~200
IS: 1:500~2000
For best results with other assays (e.g.: DB, ELISA, etc), please determine optimal working dilution by titration test.
Other Applications Image 1 Datauntransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Publications

Ablation of Ggnbp2 impairs meiotic DNA double-strand break repair during spermatogenesis in mice. J Cell Mol Med. 22, 4863-4874 (2018). 30055035

Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors. J Biol Chem. 290, 15909-20 (2015). 25944910

Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. J. Biol. Chem. 291, 2904-16 (2016). 26644464

Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and nucleotide preference. Curr Genet. 64, 1287-1301 (2018) 29761210

JiHong Li, et al. Evidence That VirS Is a Receptor for the Signaling Peptide of the Clostridium perfringens Agr-like Quorum Sensing System. (2020). 32934089

Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions. Sci Rep. 6, 23623 (2016). 27009566

Leitman, J., Ulrich Hartl, F. & Lederkremer, G. Z. Soluble forms of polyQ-expanded huntingtin rather than large aggregates cause endoplasmic reticulum stress. Nat. Commun. 4, 2753 (2013). 24217578

The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein. Int J Mol Sci. 22 (2021). 34884915

The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS. J Biol Chem. 293, 7754-7765 (2018). 29610278

Description
Alias SymbolsHis Tag
  1. What is the species homology for "His Tag Antibody (OAEA00010)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "".

  2. How long will it take to receive "His Tag Antibody (OAEA00010)"?

    This item is available "Domestic: within 5-7 days | International: 1 week".

  3. What buffer format is "His Tag Antibody (OAEA00010)" provided in?

    This item is provided in "Liquid 10mM PBS (pH 7.2) with 0.05% sodium azide".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "His Tag Antibody (OAEA00010)"?

    This target may also be called "His Tag" in publications.

  5. What is the shipping cost for "His Tag Antibody (OAEA00010)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "His Tag Antibody (OAEA00010)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "His Tag Antibody (OAEA00010)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "His Tag Antibody (OAEA00010)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with ""?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with ""?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with ""?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with ""?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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