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| Datasheets/Manuals | Printable datasheet for HIF-1 Alpha Antibody (OAAF05788) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
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| Product Format | Liquid. Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HIF-1alpha. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen. |
| Concentration | 1 mg/ml |
| Specificity | HIF-1alpha antibody detects endogenous levels of HIF-1alpha. |
| Application Info | WB: 1:500 - 1:1000 IHC: 1:50 - 1:100 ELISA: 1:10000 |
| Gene Symbol | HIF1A |
|---|---|
| Gene Full Name | hypoxia inducible factor 1 subunit alpha |
| Alias Symbols | ARNT interacting protein;ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1-alpha;hypoxia-inducible factor1alpha;member of PAS protein 1;member of PAS superfamily 1;MOP1;PAS domain-containing protein 8;PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1;PASD8. |
| NCBI Gene Id | 3091 |
| Protein Name | Hypoxia-inducible factor 1-alpha |
| Description of Target | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. |
| Uniprot ID | Q16665 |
| Molecular Weight | 92 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HIF-1 Alpha Antibody (OAAF05788)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "HIF-1 Alpha Antibody (OAAF05788)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HIF-1 Alpha Antibody (OAAF05788)" provided in?
This item is provided in "Liquid. Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HIF-1 Alpha Antibody (OAAF05788)"?
This target may also be called "ARNT interacting protein;ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1-alpha;hypoxia-inducible factor1alpha;member of PAS protein 1;member of PAS superfamily 1;MOP1;PAS domain-containing protein 8;PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1;PASD8." in publications.
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What is the shipping cost for "HIF-1 Alpha Antibody (OAAF05788)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HIF-1 Alpha Antibody (OAAF05788)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HIF-1 Alpha Antibody (OAAF05788)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "92 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HIF-1 Alpha Antibody (OAAF05788)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HIF1A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HIF1A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HIF1A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HIF1A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HIF1A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HIF1A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
