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Catalog No: OKCD00227 (Formerly GWB-KBBEP9)
Size:96WELLS
Price: $625.00
SKU
OKCD00227
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Datasheets/ManualsPrintable datasheet for HEXA ELISA Kit (Mouse) (OKCD00227)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%89-105%82-102%79-101%
EDTA plasma(n=5)92-101%83-98%95-103%79-103%
heparin plasma(n=5)92-105%79-98%87-105%94-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexosaminidase A Alpha (HEXa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexosaminidase A Alpha (HEXa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexosaminidase A Alpha (HEXa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hexosaminidase A Alpha (HEXa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-10386
EDTA plasma(n=5)96-104101
heparin plasma(n=5)96-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexosaminidase A Alpha (HEXa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexosaminidase A Alpha (HEXa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Hexa Microplate96 Wells (12 x 8 Well Strips)
Hexa Lyophilized Standard2 x 10 ng
100X Biotinylated Hexa Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hexosaminidase A Alpha (HEXa). No significant cross-reactivity or interference between Hexosaminidase A Alpha (HEXa) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHexa
Gene Full Namehexosaminidase A
Alias Symbolsbeta-hexosaminidase subunit alpha;beta-N-acetylhexosaminidase subunit alpha;Hex-1;hexosaminidase subunit A;N-acetyl-beta-glucosaminidase subunit alpha.
NCBI Gene Id15211
Protein NameBeta-hexosaminidase subunit alpha
Description of TargetResponsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Uniprot IDP29416
Protein Accession #NP_034551
Nucleotide Accession #NM_010421.4
Protein Size (# AA)528
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