Hemoglobin ELISA Kit (Human) : 96 Wells (OKCA00094)

Catalog No: OKCA00094

Size:96 Wells

Human Hemoglobin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	84%	80-92%
  Serum (n=4)	1:2	93%	89-97%
  Serum (n=4)	1:4	90%	88-94%
  Serum (n=4)	1:8	103%	96-108%

• ELISA Kit Principle: Aviva Systems Biology Hemoglobin ELISA Kit (Human) (OKCA00094) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Hemoglobin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hemoglobin and incubated. The Hemoglobin found in the sample or standards competes with the biotinylated Hemoglobin for limited binding sites on the immobilized anti-human Hemoglobin antibody. Excess unbound biotinylated Hemoglobin and sample or standard Hemoglobin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hemoglobin captured in the well and inversely proportional to the amount of Hemoglobin which was contained in the sample or standard.
• ELISA Kit Range: 15.6 ng/mL - 1,000 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n = 5)	0.95	89-98%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA plasma (n = 4)	0.98	93-103%

• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • Anti-Hemoglobin Microplate: 96 Well (12 x 8 Well Strips)
  • Hemoglobin Standard: 1 x 100 uL
  • 100X Hemoglobin-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Lysate for RBC
• Sensitivity: 7.8 ng/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of human
  Hb. No significant cross-reactivity or interference between human Hb and
  analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: Hemoglobin