HDL ELISA Kit (Human) (OKEH00700)

Catalog No: OKEH00700

Size:96T

Human HDL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HDL ELISA Kit (Human) (OKEH00700) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HDL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HDL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HDL captured in well.
• ELISA Kit Range: 125-8000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • HDL Microplate: 96 Wells (12 x 8 Well strips)
  • HDL Lyophilized Standard: 2
  • 100X Biotinylated HDL Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 61 pg/mL
• Specificity: Natural and recombinant Human High-density lipoprotein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for HDL ELISA Kit (Human) (OKEH00700) on CiteAb

Target Info

• Gene Symbol: HDL
• Alias Symbols: High-density lipoprotein
• Protein Name: High-density lipoprotein
• Description of Target: High-density lipoprotein (HDL) is one of the five major groups of lipoproteins, which, in order of sizes, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, which enable lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. In healthy individuals, about thirty percent of blood cholesterol is carried by HDL.