HDGF ELISA Kit (Human) (OKCD00249)

Catalog No: OKCD00249 (Formerly GWB-KBBOX6)

Size:96WELLS

Human HDGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-90%	78-89%	88-104%	80-101%
  EDTA plasma(n=5)	97-105%	80-101%	78-91%	82-96%
  heparin plasma(n=5)	79-103%	89-104%	85-94%	84-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatoma Derived Growth Factor (HDGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatoma Derived Growth Factor (HDGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatoma Derived Growth Factor (HDGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepatoma Derived Growth Factor (HDGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	97-104	101
  EDTA plasma(n=5)	98-105	101
  heparin plasma(n=5)	93-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatoma Derived Growth Factor (HDGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatoma Derived Growth Factor (HDGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HDGF Microplate: 96 Wells (12 x 8 Well Strips)
  • HDGF Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HDGF Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatoma Derived Growth Factor (HDGF). No significant cross-reactivity or interference between Hepatoma Derived Growth Factor (HDGF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HDGF
• Gene Full Name: heparin binding growth factor
• Alias Symbols: epididymis secretory sperm binding protein;hepatoma derived growth factor;hepatoma-derived growth factor;high mobility group protein 1-like 2;HMG1L2.
• NCBI Gene Id: 3068
• Protein Name: Hepatoma-derived growth factor
• Description of Target: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
• Uniprot ID: P51858
• Protein Accession #: NP_001119522
• Nucleotide Accession #: NM_001126050.1
• Protein Size (# AA): 240