HDAC1 ELISA Kit (Rat) (OKEH03240)

Catalog No: OKEH03240

Size:96T

Rat HDAC1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
• ELISA Kit Principle: Aviva Systems Biology HDAC1 ELISA Kit (Rat) (OKEH03240) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hdac1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hdac1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hdac1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-Assay CV: ≤4.2% (n=20)
  Inter-Assay CV: ≤8.1% (n=24)
• ELISA Kit Component:
  • Anti-Hdac1 Microplate: 96 Wells (12 x 8 Well strips)
  • Hdac1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Hdac1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.081 ng/mL
• Specificity: Natural and recombinant Rat Histone deacetylase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hdac1
• Gene Full Name: histone deacetylase 1
• Alias Symbols: HD1;histone deacetylase 1.
• NCBI Gene Id: 297893
• Protein Name: Q4QQW4
• Description of Target: Histone deacetylase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q4QQW4
• Protein Accession #: NP_001020580.1
• Nucleotide Accession #: NM_001025409.1
• Protein Size (# AA): 482
• Molecular Weight: 55 kDa