Hcrt ELISA Kit (Mouse) : 96 Wells (OKEH02770)

Catalog No: OKEH02770

Size:96T

Mouse Hcrt ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Hcrt ELISA Kit (Mouse) (OKEH02770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hcrt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hcrt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hcrt captured in well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 9.5% (n = 20)
• ELISA Kit Component:
  • Anti-Hcrt Microplate: 96 Wells (12 x 8 Well strips)
  • Hcrt Lyophilized Standard: 2
  • 100X Biotinylated Hcrt Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 4.24 pg/mL
• Specificity: Natural and recombinant Mouse Orexin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hcrt
• Gene Full Name: hypocretin
• Alias Symbols: Hypocretin;orexin;orexin A;orexin B;PPOX;prepro-orexin.
• NCBI Gene Id: 15171
• Protein Name: O55241
• Description of Target: Orexin
• Uniprot ID: https://www.uniprot.org/uniprot/O55241
• Protein Accession #: NP_034540.1
• Nucleotide Accession #: NM_010410.2
• Protein Size (# AA): 130
• Molecular Weight: 14 kDa