Catalog No: OKEH02769
Size:96T
Price: $525.00
SKU
OKEH02769
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HCRT ELISA Kit (Human) : 96 Wells (OKEH02769)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HCRT ELISA Kit (Human) (OKEH02769) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HCRT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HCRT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HCRT captured in well.
ELISA Kit Range9.375-600pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.7% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-HCRT Microplate96 Wells (12 x 8 Well strips)
HCRT Lyophilized Standard2
100X Biotinylated HCRT Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Orexin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHCRT
Gene Full Namehypocretin neuropeptide precursor
Alias SymbolsHypocretin;hypocretin (orexin) neuropeptide;NRCLP1;orexin;OX;PPOX;prepro-orexin.
NCBI Gene Id3060
Protein NameO43612
Description of TargetOrexin
Uniprot IDhttps://www.uniprot.org/uniprot/O43612
Protein Accession #NP_001515.1
Nucleotide Accession #NM_001524.1
Protein Size (# AA)131
Molecular Weight14 kDa
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