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Datasheets/Manuals | Printable datasheet for anti-HCK (OASG03286) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived from human Hck around the phosphorylation site of Y521. Location: 450-530aa. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Tyr521 |
Specificity | Phospho-Hck (Tyr521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521. |
Application Info | WB 1:500-1:2000; ELISA 1:10000; |
Gene Symbol | HCK |
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Gene Full Name | HCK proto-oncogene, Src family tyrosine kinase |
Alias Symbols | JTK9, p59Hck, p61Hck |
NCBI Gene Id | 3055; 15162; 25734 |
Description of Target | The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. |
Uniprot ID | P08631, P08103, P50545 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "HCK Antibody (Phospho-Tyr521) (OASG03286)" provided in?
This item is provided in "Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
This target may also be called "JTK9, p59Hck, p61Hck" in publications.
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What is the shipping cost for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "60 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HCK Antibody (Phospho-Tyr521) (OASG03286)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HCK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HCK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HCK"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HCK"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HCK"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HCK"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.