HCK Antibody (OAAB21303)

Catalog No: OAAB21303

Size:200ul

All lanes: Anti-HCK Antibody (OAAB21303) at 1:2,000-1:4,000 dilution Lane 1:THP-1 whole cell lysates Lane 2: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with OAAB21303 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 1508CT602.13.1
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB, IF
  Cellular Location: Isoform 1: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Note=Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.
  Tissue Location: Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes Detected in tonsil.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HCK antibody is generated from a mouse immunized with a recombinant protein of human HCK.
• Purification: Protein G column purification, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 500 ug/mL
• Application Info: IF: 1:25
  WB: 1:2000~1:4000
• Intended Use: HCK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Reference: Quintrell N.,et al.Mol. Cell. Biol. 7:2267-2275(1987).
  Ziegler S.F.,et al.Mol. Cell. Biol. 7:2276-2285(1987).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Deloukas P.,et al.Nature 414:865-871(2001).
  Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: HCK
• Gene Full Name: HCK proto-oncogene, Src family tyrosine kinase
• Alias Symbols: JTK9, p59Hck, p61Hck
• NCBI Gene Id: 3055
• Description of Target: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
• Uniprot ID: P08631
• Protein Accession #: NP_001165600.1
• Nucleotide Accession #: NM_001172129.1
• Molecular Weight: 59600 Da