HCFC1 Antibody - middle region (ARP38601_P050)

Catalog No: ARP38601_P050

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WB Suggested Anti-HCFC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Stomach

Lanes:
Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate
Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit AlexaFluor 680
Secondary Antibody Dilution:
1:10000
Gene Name:
HCFC1
Submitted by:
Anonymous

Hum. Fetal Heart

Hum. Fetal Liver

Host: Rabbit
Target Name: HCFC1
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: HCFC1
Positive control (+): Human Stomach (ST)
Negative control (-): Human Small Intestine (IN)
Antibody concentration: 1ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HCFC1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADG
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-HCFC1 (ARP38601_P050) antibody is Catalog # AAP38601 (Previous Catalog # AAPP20790)
• Reference: Vrabec,J.T., (2008) Otol. Neurotol. 29 (4), 561-566

Target Info

• Gene Symbol: HCFC1
• Gene Full Name: Host cell factor C1 (VP16-accessory protein)
• Alias Symbols: CFF, HCF, HCF1, HFC1, MRX3, VCAF, HCF-1, PPP1R89
• NCBI Gene Id: 3054
• Protein Name: Host cell factor 1
• Description of Target: HCFC1 is a member of the host cell factor family with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possib
• Uniprot ID: P51610
• Protein Accession #: NP_005325
• Nucleotide Accession #: NM_005334
• Protein Size (# AA): 2035
• Molecular Weight: 209kDa
• Protein Interactions: FBXW11; BAP1; NUDCD3; CREB3; UBC; RPA3; RPA2; RPA1; EED; EEF1D; CREBZF; LMNA; OGT; POU2F1; NOTCH1; UL48; RBBP5; PPP1CB; PPP1CA; DPY30; WDR5; MAP3K7; HCFC1; EGFR; CLP1; YEATS2; CDK2; CUL3; USF1; NR2C1; THAP11; SIRT7; ELAVL1; SUMO2; ASH2L; SNRPN; SNRPB; YY1