HBEGF ELISA Kit (Human) (OKBB00436)

Catalog No: OKBB00436

Size:96 Wells

Human HBEGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(NS0, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human HBEGF amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	92.7	369	621	102	376	618
  SD	5.1	16.97	23.6	8.26	27.82	42.02
  CV%	5.5	4.6	3.8	8.1	7.4	6.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human HBEGF Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human HBEGF Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human HBEGF Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human HBEGF
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D63-L148
• Description: For quantitative detection of human HBEGF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: HBEGF
• Gene Full Name: heparin-binding EGF-like growth factor
• Alias Symbols: Diphtheria toxin receptor, diphtheria toxin receptor (heparin-binding EGF-like growth factor), diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), DT R, DT-R, DTR, DTS, DTSF, HB-EGF, HBEGF, HBEGF_HUMAN, HEGFL, Heparin binding EGF like growth factor, Heparin binding epidermal growth factor, Heparin binding epidermal growth factor like growth factor, Heparin-binding EGF-like growth factor, Proheparin binding EGF like growth factor
• NCBI Gene Id: 1839
• Protein Name: Proheparin-binding EGF-like growth factor
• Description of Target: HBEGF (Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
• Uniprot ID: Q99075
• Protein Accession #: NP_001936.1
• Nucleotide Accession #: NM_001945.2