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Catalog No: OKCD00688 (Formerly GWB-KBBKL2)
Size:96WELLS
Price: $675.00
SKU
OKCD00688
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Datasheets/ManualsPrintable datasheet for HBA1 MORE ELISA Kit (Human) (OKCD00688)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-95%79-102%88-104%90-98%
EDTA plasma(n=5)80-91%79-105%80-99%91-101%
heparin plasma(n=5)90-98%87-101%86-94%81-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Alpha 1 (HBa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Alpha 1 (HBa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin Alpha 1 (HBa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hemoglobin Alpha 1 (HBa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-10193
EDTA plasma(n=5)81-9892
heparin plasma(n=5)91-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HBA1 more Microplate96 Wells (12 x 8 Well Strips)
HBA1 more Lyophilized Standard2 x 100 ng
100X Biotinylated HBA1 more Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, erythrocyte lysates and other biological fluids.
Sensitivity< 0.63 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hemoglobin Alpha 1 (HBa1). No significant cross-reactivity or interference between Hemoglobin Alpha 1 (HBa1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHBA1|HBA2
Gene Full Namehemoglobin subunit alpha 1|hemoglobin subunit alpha 2
Alias Symbolsalpha globin;alpha globin chain;alpha one globin;alpha-2 globin;alpha-2 globin chain;Alpha-globin;delta globin;ECYT7;HBA-T2;HBA-T3;HBH;hemoglobin alpha 1 globin chain;hemoglobin alpha chain;hemoglobin subunit alpha;hemoglobin, alpha 1;hemoglobin, alpha 2;METHBA;mutant hemoglobin alpha 2 globin chain;truncated HbA2.
NCBI Gene Id3039|3040
Protein NameHemoglobin subunit alpha
Description of TargetInvolved in oxygen transport from the lung to the various peripheral tissues.
Uniprot IDP69905
Protein Accession #NP_000508
Nucleotide Accession #NM_000517.4
Protein Size (# AA)142
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