HARS Antibody - N-terminal region (ARP61337_P050)

Catalog No: ARP61337_P050

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WB Suggested Anti-HARS Antibody
Titration: 1.0 ug/ml
Positive Control: 293T Whole CellHARS is supported by BioGPS gene expression data to be expressed in HEK293T

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HARS
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: FVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETL
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-HARS (ARP61337_P050) antibody is Catalog # AAP61337 (Previous Catalog # AAPP47465)
• Sample Type Confirmation:

  HARS is supported by BioGPS gene expression data to be expressed in HEK293T

Target Info

• Gene Symbol: HARS
• Gene Full Name: Histidyl-tRNA synthetase
• Alias Symbols: HRS, HARS, CMT2W, USH3B
• NCBI Gene Id: 3035
• Protein Name: Histidine--tRNA ligase, cytoplasmic
• Description of Target: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
• Uniprot ID: P12081
• Protein Accession #: NP_002100
• Nucleotide Accession #: NM_002109
• Protein Size (# AA): 509
• Molecular Weight: 56kDa
• Protein Interactions: UBC; AARSD1; ACBD3; ELAC2; XPO5; THG1L; NSUN2; RPUSD2; SNF8; IPO7; EFTUD2; TTC1; SHMT1; PFDN1; GTF2E1; FEN1; CALU; PI4KA; G6PD; EIF2D; ANXA5; UBD; SUMO1; TNIK; STAM; EIF3I; EEF1G; RPL5; EEF1B2; TARS;