HACE1 ELISA Kit (Mouse) (OKEH06783)

Catalog No: OKEH06783

Size:96T

Mouse HACE1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Hace1 ELISA Kit (Mouse) (OKEH06783) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hace1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hace1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hace1 captured in the well.
• ELISA Kit Range: 0.78-50ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into serum = 104.5%
• ELISA Kit Reproducibility: Intra-Assay Reproducibility CV% < 4.6% (n=20)< br>Inter-Assay Reproducibility CV% < 7.4% (n=24)
• ELISA Kit Component:
  • NRGN Microplate: 96 Wells (12 x 8 Well strips)
  • NRGN Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated NRGN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hace1
• Gene Full Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
• Alias Symbols: 1700042J16Rik;A730034A22Rik;BC025474;E3 ubiquitin-protein ligase HACE1;HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1;HECT-type E3 ubiquitin transferase HACE1.
• NCBI Gene Id: 209462
• Protein Name: Q3U0D9
• Description of Target: E3 ubiquitin-protein ligase HACE1
• Uniprot ID: https://www.uniprot.org/uniprot/Q3U0D9
• Protein Accession #: NP_766061
• Nucleotide Accession #: NM_172473