HABP2 ELISA Kit (Human) (OKCD01178)

Catalog No: OKCD01178 (Formerly GWB-KBBIT7)

Size:96WELLS

Human HABP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-92%	84-91%	79-97%	82-93%
  EDTA plasma(n=5)	78-98%	90-97%	94-101%	90-97%
  heparin plasma(n=5)	98-105%	96-105%	99-105%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Binding Protein 2 (HABP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Binding Protein 2 (HABP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Binding Protein 2 (HABP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hyaluronan Binding Protein 2 (HABP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-101	94
  EDTA plasma(n=5)	89-103	95
  heparin plasma(n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Binding Protein 2 (HABP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Binding Protein 2 (HABP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HABP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • HABP2 Lyophilized Standard: 2 x 400 ng
  • 100X Biotinylated HABP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 2.53 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hyaluronan Binding Protein 2 (HABP2). No significant cross-reactivity or interference between Hyaluronan Binding Protein 2 (HABP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HABP2
• Gene Full Name: hyaluronan binding protein 2
• Alias Symbols: factor seven-activating protease;factor VII activating protein;factor VII-activating protease;FSAP;HABP;hepatocyte growth factor activator-like protein;HGFAL;hyaluronan-binding protein 2;hyaluronic acid binding protein 2;NMTC5;PHBP;plasma hyaluronan binding protein;Plasma hyaluronan-binding protein.
• NCBI Gene Id: 3026
• Protein Name: Hyaluronan-binding protein 2
• Description of Target: Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII (PubMed:8827452, PubMed:10754382, PubMed:11217080). May function as a tumor suppressor negatively regulating cell proliferation and cell migration (PubMed:26222560).
• Uniprot ID: Q14520
• Protein Accession #: NP_001171131
• Nucleotide Accession #: NM_001177660.2
• Protein Size (# AA): 560