H2AFV ELISA Kit (Human) (OKCD00713)

Catalog No: OKCD00713 (Formerly GWB-KBBLG2)

Size:96WELLS

Human H2AFV ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member V (H2AFV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member V (H2AFV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member V (H2AFV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of H2A Histone Family, Member V (H2AFV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2A Histone Family, Member V (H2AFV) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2A Histone Family, Member V (H2AFV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-H2AFV Microplate: 96 Wells (12 x 8 Well Strips)
  • H2AFV Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated H2AFV Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member V (H2AFV). No significant cross-reactivity or interference between H2A Histone Family, Member V (H2AFV) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: H2AZ2
• Gene Full Name: H2A.Z variant histone 2
• Alias Symbols: H2A histone family member V;H2A.F/Z;H2A.Z-2;H2AFV;H2AV;histone H2A.F/Z;histone H2A.V;purine-rich binding element protein B.
• NCBI Gene Id: 94239
• Protein Name: Histone H2A.V
• Description of Target: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division (By similarity).
• Uniprot ID: Q71UI9
• Protein Accession #: NP_036544
• Nucleotide Accession #: NM_012412.4
• Protein Size (# AA): 128