Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02479
Size:96T
Price: $625.00
SKU
OKEH02479
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology H2AA ELISA Kit (Mouse) (OKEH02479) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse H2AA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H2AA and incubated. The H2AA found in the sample or standards competes with the biotinylated H2AA for limited binding sites on the immobilized anti-Mouse H2AA antibody. Excess unbound biotinylated H2AA and sample or standard H2AA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H2AA captured in the well and inversely proportional to the amount of H2AA which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-H2AA Microplate96 Wells (12 x 8 Well strips)
H2AA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X H2AA Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.325 ng/mL
SpecificityNatural and recombinant Mouse H-2 class II histocompatibility antigen, A-B alpha chain
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolH2-Aa
Gene Full Namehistocompatibility 2, class II antigen A, alpha
Alias SymbolsA;Aalpha;H-2;H-2 class II histocompatibility antigen, A-B alpha chain;H-2 class II histocompatibility antigen, A-F alpha chain;H-2 class II histocompatibility antigen, A-K alpha chain;H-2 class II histocompatibility antigen, A-Q alpha chain;H-2 class II histocompatibility antigen, A-R alpha chain;H-2 class II histocompatibility antigen, A-S alpha chain;H-2 class II histocompatibility antigen, A-U alpha chain;H2Aa;H-2Aa;histocompatibility 2, class II antigen A, alpha;histocompatibility 2, class II antigen A, alpha, promoter 1;I;I-;Ia;Ia1;Ia-1;IAalpha;I-Aalpha;major histocompatibility protein class II alpha chain;MHC class II antigen alpha chain;MHC-II I-A(d) alpha.
NCBI Gene Id14960
Protein NameP14434
Description of TargetH-2 class II histocompatibility antigen, A-B alpha chain
Uniprot IDhttps://www.uniprot.org/uniprot/P14434
Protein Accession #NP_034508.2
Nucleotide Accession #NM_010378.2
Protein Size (# AA)256
Molecular Weight28 kDa
Write Your Own Review
You're reviewing:H2AA ELISA Kit (Mouse) (OKEH02479)
Your Rating