Catalog No: OKEH02479
Size:96T
Price: $625.00
SKU
OKEH02479
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology H2AA ELISA Kit (Mouse) (OKEH02479) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse H2AA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H2AA and incubated. The H2AA found in the sample or standards competes with the biotinylated H2AA for limited binding sites on the immobilized anti-Mouse H2AA antibody. Excess unbound biotinylated H2AA and sample or standard H2AA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H2AA captured in the well and inversely proportional to the amount of H2AA which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.3% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.325 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse H-2 class II histocompatibility antigen, A-B alpha chain | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | H2-Aa |
---|---|
Gene Full Name | histocompatibility 2, class II antigen A, alpha |
Alias Symbols | A;Aalpha;H-2;H-2 class II histocompatibility antigen, A-B alpha chain;H-2 class II histocompatibility antigen, A-F alpha chain;H-2 class II histocompatibility antigen, A-K alpha chain;H-2 class II histocompatibility antigen, A-Q alpha chain;H-2 class II histocompatibility antigen, A-R alpha chain;H-2 class II histocompatibility antigen, A-S alpha chain;H-2 class II histocompatibility antigen, A-U alpha chain;H2Aa;H-2Aa;histocompatibility 2, class II antigen A, alpha;histocompatibility 2, class II antigen A, alpha, promoter 1;I;I-;Ia;Ia1;Ia-1;IAalpha;I-Aalpha;major histocompatibility protein class II alpha chain;MHC class II antigen alpha chain;MHC-II I-A(d) alpha. |
NCBI Gene Id | 14960 |
Protein Name | P14434 |
Description of Target | H-2 class II histocompatibility antigen, A-B alpha chain |
Uniprot ID | https://www.uniprot.org/uniprot/P14434 |
Protein Accession # | NP_034508.2 |
Nucleotide Accession # | NM_010378.2 |
Protein Size (# AA) | 256 |
Molecular Weight | 28 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review