GZMH ELISA Kit (Human) (OKCD00727)

Catalog No: OKCD00727 (Formerly GWB-KBBMK1)

Size:96WELLS

Human GZMH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-94%	86-93%	97-104%	78-91%
  EDTA plasma(n=5)	88-102%	78-90%	79-102%	98-105%
  heparin plasma(n=5)	92-105%	90-98%	83-95%	92-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme H (GZMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme H (GZMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme H (GZMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Granzyme H (GZMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-90	87
  EDTA plasma(n=5)	88-96	93
  heparin plasma(n=5)	91-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granzyme H (GZMH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granzyme H (GZMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GZMH Microplate: 96 Wells (12 x 8 Well Strips)
  • GZMH Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated GZMH Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.61 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Granzyme H (GZMH). No significant cross-reactivity or interference between Granzyme H (GZMH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GZMH
• Gene Full Name: granzyme H
• Alias Symbols: Cathepsin G-like 2;cathepsin G-like 2, protein h-CCPX;CCP-X;CGL-2;CSP-C;CTLA1;CTSGL2;Cytotoxic serine protease C;cytotoxic T-lymphocyte proteinase;cytotoxic T-lymphocyte-associated serine esterase 1;cytotoxin serine protease-C;granzyme H.
• NCBI Gene Id: 2999
• Protein Name: Granzyme H
• Description of Target: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
• Uniprot ID: P20718
• Protein Accession #: NP_001257710
• Nucleotide Accession #: NM_001270781.1
• Protein Size (# AA): 246