GSK3B Antibody - C-terminal region (ARP54644_P050)

Catalog No: ARP54644_P050

WB Suggested Anti-GSK3B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateGSK3B is supported by BioGPS gene expression data to be expressed in 721_B

Host: Rabbit
Target Name: GSK3B
Sample Type: Hela
Antibody Dilution: 1.0ug/mlGSK3B is supported by BioGPS gene expression data to be expressed in HeLa

Host: Rabbit
Target Name: GSK3B
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: GSK3B
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: GSK3B
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml

Brain, cortex

Rabbit Anti-GSK3B Antibody
Catalog Number: ARP54644_P050
Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
Observed Staining: Cytoplasm, Nucleus, Plasma membrane
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: Paraffin embedded brain, cortex tissue, tested with an antibody dilution of 2.5 ug/ml.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GSK3B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: AIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNF
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-GSK3B (ARP54644_P050) antibody is Catalog # AAP54644 (Previous Catalog # AAPP31435)
• Sample Type Confirmation:

  GSK3B is supported by BioGPS gene expression data to be expressed in 721_B, HeLa

• Reference: Izumi,N., (2008) J. Biol. Chem. 283 (19), 12981-12991

Target Info

• Gene Symbol: GSK3B
• Gene Full Name: Glycogen synthase kinase 3 beta
• Alias Symbols: -
• NCBI Gene Id: 2932
• Protein Name: Glycogen synthase kinase-3 beta
• Description of Target: Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P49841
• Protein Accession #: NP_002084
• Nucleotide Accession #: NM_002093
• Protein Size (# AA): 433
• Molecular Weight: 48kDa
• Protein Interactions: AKT1; MAP3K4; MAPT; JUN; RNF220; HDAC4; AXIN1; SNAI2; NBR1; AURKA; UBC; axl-1; pry-1; PRKAR1A; EYA1; GSK3B; AKAP11; BTRC; CTNNB1; HECW2; PRKACA; PPP1R2; APC; FRAT1; AXIN2; GSKIP; LRP6; CSF3R; DYNLL1; VIM; FRAT2; EPB41L3; CLASP2; HSP90AA1; PPP1CA; PRUNE; N