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Catalog No: OKEH03020
Size:96T
Price: $475.00
SKU
OKEH03020
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GSK3A ELISA Kit (Rat) (OKEH03020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gsk3a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gsk3a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gsk3a captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Gsk3a Microplate96 Wells (12 x 8 Well strips)
Gsk3a Lyophilized Standard2
100X Biotinylated Gsk3a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.161 ng/mL
SpecificityNatural and recombinant Rat Glycogen synthase kinase-3 alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGsk3a
Gene Full Nameglycogen synthase kinase 3 alpha
Alias SymbolsFA;factor A;glycogen synthase kinase-3 alpha;GSK-3 alpha;serine/threonine-protein kinase GSK3A.
NCBI Gene Id50686
Protein NameP18265
Description of TargetGlycogen synthase kinase-3 alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P18265
Protein Accession #NP_059040.1
Nucleotide Accession #NM_017344.1
Protein Size (# AA)483
Molecular Weight51 kDa
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