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Catalog No: OKBB00284
Size:96 Tests
Price: $583.00
SKU
OKBB00284
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Growth Hormone ELISA Kit (Human) (OKBB00284)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human GH1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH1 has been precoated onto 96-well plates. Standards(E.coli, F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human GH1 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Component1. Lyophilized recombinant human E-Cadherin standard: 10ng/tubex2.2. One 96-well plate precoated with anti-human E-Cadherin antibody.3. Sample diluent buffer: 30 ml4. Biotinylated anti-human E-Cadherin antibody : 130ul, dilution 1:100.5. Antibody diluent buffer: 12ml.6. Avidin-Biotin-Peroxidase Complex 7. ABC diluent buffer: 12ml.(ABC): 130ul, dilution 1:100.8. TMB color developing agent: 10ml.,9. TMB stop solution: 10ml.
Additional InformationRange: 15.6pg/ml-1000pg/ml
::Principle: Aviva’s human Growth Hormone ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Growth Hormone specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Growth Hormone amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity<2 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine
Reference1. Baum, H. B. A.; Katznelson, L.; Sherman, J. C.; Biller, B. M. K.; Hayden, D. L.; Schoenfeld, D. A.; Cannistraro, K. E.;
Klibanski, A. : Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with
adult-onset GH deficiency. J. Clin. Endocr. Metab. 83: 3184-3189, 1998
2. Niall, H. D.; Hogan, M. L.; Sauer, R.; Rosenblum, I. Y.; Greenwood, F. C. :
Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene
reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971.
3. Owerbach, D.; Rutter, W. J.; Martial, J. A.; Baxter, J. D.; Shows, T. B. : Genes for growth hormone, chorionic
somatomammotropin and growth hormone-like genes on chromosome 17 in humans. Science 209: 289-292, 1980.
SpecificityNatural and recombinant human GH1
ImmunogenExpression system for standard: E.coli; Immunogen sequence: F27-F217
DescriptionFor quantitative detection of human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolGH1
Gene Full Namegrowth hormone 1
Alias SymbolsgH, GH-N, GH1, GHN, Growth hormone, Growth hormone 1, HGH, Pituitary growth hormone, SOMA_HUMAN, Somatotropin
NCBI Gene Id2688
Protein NameSomatotropin
Description of TargetGrowth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life.
Uniprot IDP01241
Protein Accession #NP_000506.2
Nucleotide Accession #NM_000515.4
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