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Catalog No: OKCD01282
Size:96WELLS
Price: $800.00
SKU
OKCD01282
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Growth Hormone Antibody ELISA Kit (Human) (OKCD01282)

Datasheets/ManualsPrintable datasheet for Growth Hormone Antibody ELISA Kit (Human) (OKCD01282)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h, 30min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%80-97%79-88%89-96%
EDTA Plasma (n=5)78-104%81-102%99-105%98-105%
Heparin Plasma (n=5)83-105%95-103%81-95%80-98%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Growth Hormone Antibody (Anti-GH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Growth Hormone Antibody (Anti-GH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-10182
EDTA Plasma (n=5)86-9895
Heparin Plasma (n=5)95-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Growth Hormone Antibody (Anti-GH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Growth Hormone Antibody (Anti-GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Growth Hormone antibody Microplate96 Wells (12 x 8 Well strips)
Growth Hormone antibody Lyophilized Standard2 x XXX
100X Detector Antibody1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.12 ng/mL
Assay InfoAssay Method: Quantitative Reverse Capture Sandwich ELISA
Gene SymbolGH1
Gene Full Namegrowth hormone 1
Alias SymbolsGH;GHB5;GHN;GH-N;Growth hormone;Growth hormone 1;growth hormone B5;hGH-N;IGHD1A;IGHD1B;IGHD2;pituitary growth hormone;somatotropin.
NCBI Gene Id2688
Protein NameSomatotropin
Description of TargetPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Uniprot IDP01241
Nucleotide Accession #NM_000515.4
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