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Catalog No: OKEH04008
Size:96T
Price: $425.00
SKU
OKEH04008
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GRN ELISA Kit (Human) (OKEH04008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRN captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-GRN Microplate96 Wells (12 x 8 Well Strips)
GRN Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
GRN Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Granulins
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGRN
Gene Full Namegranulin precursor
Alias Symbolsacrogranin;CLN11;epithelin;GEP;glycoprotein 88;glycoprotein of 88 Kda;GP88;granulin-epithelin;granulins;PC cell-derived growth factor;PCDGF;PEPI;PGRN;proepithelin;progranulin.
NCBI Gene Id2896
Protein NameP28799
Description of TargetGranulins
Uniprot IDhttps://www.uniprot.org/uniprot/P28799
Protein Accession #NP_002078.1
Nucleotide Accession #NM_002087.3
Protein Size (# AA)593
Molecular Weight64 kDa
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