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Datasheets/Manuals | Printable datasheet for GRIN2A Antibody (OAAF01066) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR2A/B. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | NMDAR2A/B antibody detects endogenous levels of total NMDAR2A/B protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000 |
Reference | Foldes R.L., Biochim. Biophys. Acta 1223:155-159(1994). Hess S.D., J. Pharmacol. Exp. Ther. 278:808-816(1996). Olsen J.V., Cell 127:635-648(2006) |
Gene Symbol | GRIN2A|GRIN2B |
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Gene Full Name | glutamate ionotropic receptor NMDA type subunit 2A|glutamate ionotropic receptor NMDA type subunit 2B |
Alias Symbols | DEE27, EIEE27, EPND, FESD, GluN2A, GluN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-1, glutamate [NMDA] receptor subunit epsilon-2, glutamate ionotropic receptor NMDA 2A, glutamate receptor ionotropic, NMDA 2A, glutamate receptor ionotropic, NMDA |
NCBI Gene Id | 2903|2904 |
Protein Name | Glutamate receptor ionotropic, NMDA 2A|Glutamate receptor ionotropic, NMDA 2B |
Description of Target | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28105280). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (PubMed:26919761, PubMed:26875626). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity).|Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28126851). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:8768735, PubMed:26875626). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity). |
Uniprot ID | Q12879|Q13224 |
Protein Accession # | NP_000825.2 |
Molecular Weight | 165 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GRIN2A Antibody (OAAF01066)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "GRIN2A Antibody (OAAF01066)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "GRIN2A Antibody (OAAF01066)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GRIN2A Antibody (OAAF01066)"?
This target may also be called "DEE27, EIEE27, EPND, FESD, GluN2A, GluN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-1, glutamate [NMDA] receptor subunit epsilon-2, glutamate ionotropic receptor NMDA 2A, glutamate receptor ionotropic, NMDA 2A, glutamate receptor ionotropic, NMDA" in publications.
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What is the shipping cost for "GRIN2A Antibody (OAAF01066)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GRIN2A Antibody (OAAF01066)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GRIN2A Antibody (OAAF01066)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "165 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GRIN2A Antibody (OAAF01066)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GRIN2A|GRIN2B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GRIN2A|GRIN2B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GRIN2A|GRIN2B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GRIN2A|GRIN2B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GRIN2A|GRIN2B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GRIN2A|GRIN2B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.