GPX3 ELISA Kit (Human) : 96 Wells (OKEH02034)

Catalog No: OKEH02034

Size:96T

Human GPX3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GPX3 ELISA Kit (Human) (OKEH02034) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GPX3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GPX3 and incubated. The GPX3 found in the sample or standards competes with the biotinylated GPX3 for limited binding sites on the immobilized anti-Human GPX3 antibody. Excess unbound biotinylated GPX3 and sample or standard GPX3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GPX3 captured in the well and inversely proportional to the amount of GPX3 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 7.8% (n = 20)
• ELISA Kit Component:
  • Anti-GPX3 Microplate: 96 Wells (12 x 8 Well strips)
  • GPX3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X TGFBR2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Glutathione peroxidase 3
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: GPX3
• Gene Full Name: glutathione peroxidase 3
• Alias Symbols: extracellular glutathione peroxidase;glutathione peroxidase 3;glutathione peroxidase 3 (plasma);GPx-3;GPx-P;GSHPx-3;GSHPx-P;plasma glutathione peroxidase;selenoprotein GPX3.
• NCBI Gene Id: 2878
• Protein Name: P22352
• Description of Target: Glutathione peroxidase 3
• Uniprot ID: https://www.uniprot.org/uniprot/P22352
• Protein Accession #: NP_002075.2
• Nucleotide Accession #: NM_002084.3
• Protein Size (# AA): 229
• Molecular Weight: 257 kDa