Catalog No: OKEH04862
Size:96T
Price: $525.00
SKU
OKEH04862
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Gpx2 ELISA Kit (Rat) (OKEH04862) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpx2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpx2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpx2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Gpx2 Microplate96 Wells (12 x 8 Well Strips)
Gpx2 Standard2
Sample Diluent1 x 20 mL
Gpx2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity39.01 pg/mL
SpecificityNatural and recombinant Rat Glutathione peroxidase 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGPX2
Gene Full NameGlutathione peroxidase 2
Alias SymbolsGlutathione peroxidase-gastrointestinal.
NCBI Gene Id29326
Protein NameP83645
Description of TargetGlutathione peroxidase 2
Uniprot IDhttps://www.uniprot.org/uniprot/P83645
Protein Accession #NP_899653.2
Nucleotide Accession #NM_183403.2
Protein Size (# AA)190
Molecular Weight22 kDa
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