GPC5 ELISA Kit (Human) (OKCA01260)

Catalog No: OKCA01260

Size:96 Wells

Human GPC5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	93	88-97
  1:2	97	92-102
  1:4	100	94-107
  1:8	108	103-114

• ELISA Kit Principle: Aviva Systems Biology GPC5 ELISA Kit (Human) (OKCA01260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPC5 captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	102	95-106
  EDTA Plasma (n=4)	95	89-104

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01260 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01260 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • GPC5 Microplate: 96 Wells (12 x 8 Well strips)
  • GPC5 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated GPC5 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Ascitic Fluid
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: GPC5
• Gene Full Name: glypican 5
• Alias Symbols: bA93M14.1|glypican proteoglycan 5
• NCBI Gene Id: 2262
• Protein Name: Glypican-5
• Description of Target: Cell surface proteoglycan that bears heparan sulfate.
• Uniprot ID: P78333
• Protein Accession #: NP_004457.1
• Nucleotide Accession #: NM_004466.5