Catalog No: OKBB00396
Size:96 Wells
Price: $583.00
SKU
OKBB00396
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human GP130 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GP130 has been precoated onto 96-well plates. Standards(NS0, L24-E619) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GP130 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human GP130 amount of sample captured in plate.
ELISA Kit Range125 pg/ml - 8,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)6081555283671519383147
SD27.9780.86167.344.33133.7229.7
CV%4.65.25.96.26.97.3
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Gp130 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Gp130 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human Gp130 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human Sgp130
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: L24-E619
DescriptionFor quantitative detection of human soluble GP130 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Gene SymbolIL6ST
Gene Full Nameinterleukin 6 signal transducer
Alias SymbolsCD130 antigen, CDw130, gp130, IL6 R beta, Interleukin 6 receptor subunit beta, Interleukin 6 signal transducer, Membrane glycoprotein 130, Oncostatin M receptor alpha subunit
NCBI Gene Id3572
Protein NameInterleukin-6 receptor subunit beta
Description of TargetGP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
Uniprot IDP40189
Protein Accession #NP_001177910.1
Nucleotide Accession #NM_001190981.1
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