GOT1 ELISA Kit (Mouse) (OKCD00822)

Catalog No: OKCD00822 (Formerly GWB-KBBSK2)

Size:96WELLS

Mouse Got1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-101%	78-92%	86-93%	99-105%
  EDTA plasma(n=5)	94-102%	92-99%	92-101%	99-105%
  heparin plasma(n=5)	84-92%	95-104%	81-101%	80-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-90	87
  EDTA plasma(n=5)	97-105	101
  heparin plasma(n=5)	96-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Got1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Got1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Got1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 13.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST). No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Got1
• Gene Full Name: glutamic-oxaloacetic transaminase 1, soluble
• Alias Symbols: AI789014;aspartate aminotransferase, cytoplasmic;cA;cAspAT;cCAT;cysteine aminotransferase, cytoplasmic;cysteine transaminase, cytoplasmic;cytosolic aspartate aminotransferase;Glutamate oxaloacetate transaminase 1;glutamate oxaloacetate transaminase 1, soluble;Got-;Got-1;transaminase A.
• NCBI Gene Id: 14718
• Protein Name: Aspartate aminotransferase, cytoplasmic
• Description of Target: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).
• Uniprot ID: P05201
• Protein Accession #: NP_034454
• Nucleotide Accession #: NM_010324.2
• Protein Size (# AA): 413