GOT1 ELISA Kit (Human) (OKCD02389)

Catalog No: OKCD02389

Size:96WELLS

Human GOT1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-101%	94-102%	81-89%	87-101%
  EDTA Plasma (n=5)	80-103%	89-98%	86-94%	79-101%
  Heparin Plasma (n=5)	83-97%	97-105%	93-101%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	102
  EDTA Plasma (n=5)	84-103	101
  Heparin Plasma(n=5)	95-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GOT1 Microplate: 96 Wells (12 x 8 Well strips)
  • GOT1 Lyophilized Standard: 2
  • 100X Biotinylated GOT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.119 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST). No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GOT1
• Gene Full Name: glutamic-oxaloacetic transaminase 1
• Alias Symbols: aspartate aminotransferase 1;aspartate aminotransferase, cytoplasmic;aspartate transaminase 1;AST1;ASTQTL1;cAspAT;cCAT;cysteine aminotransferase, cytoplasmic;cysteine transaminase, cytoplasmic;GIG18;glutamate oxaloacetate transaminase 1;glutamic-oxaloacetic transaminase 1, soluble;growth-inhibiting protein 18;testis secretory sperm-binding protein Li 196a;transaminase A.
• NCBI Gene Id: 2805
• Protein Name: Aspartate aminotransferase, cytoplasmic
• Description of Target: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
• Uniprot ID: P17174
• Protein Accession #: NP_002070.1
• Nucleotide Accession #: NM_002079.2
• Protein Size (# AA): 413