Goat Anti-WHIP / WRNIP1 Antibody (OAEB01035)

Catalog No: OAEB01035 (Formerly GWB-E59285)

Size:100UG

OAEB01035 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: C Terminus
  Domains: ATP synthase alpha/beta family, nucleotide-binding domain; ATPase family associated with various cellular activities (AAA); ATPases associated with a variety of cellular activities; Bacterial dnaA protein; DEAD-like helicases
  Note: This antibody is expected to recognise both reported isoforms according to NP_569079.1 and NP_064520.2.
  Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Pig, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: WRNIP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 92%
• Peptide Sequence: EELRGVDFFKQRRC
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32000.
  Western Blot: Preliminary experiments gave an approx 45kDa band in human lung and brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of 72kDa for isoform 1 according to NP_064520.2 and 69.5kDa for isoform 2 according to NP_569079.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T.
  A novel protein interacts with the Werner's syndrome gene product physically
  and functionally.
  J Biol Chem. 2001 Jun 8;276(23):20364-9. Epub 2001 Apr 11. PMID: 11301316

Target Info

• Gene Symbol: WRNIP1
• Gene Full Name: WRN helicase interacting protein 1
• Alias Symbols: ATPase WRNIP1;bA420G6.2;CFAP93;FAP93;putative helicase RUVBL;Werner helicase interacting protein 1;WHIP.
• NCBI Gene Id: 56897
• Protein Name: ATPase WRNIP1
• Uniprot ID: Q96S55
• Protein Accession #: NP_064520.2
• Molecular Weight: 72.1; 69.5