Goat Anti-USP11 / UHX1 Antibody (OAEB02172)

Catalog No: OAEB02172 (Formerly GWB-991637)

Size:100UG

OAEB02172 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: USP11
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 84%; Horse: 91%; Human: 100%; Pig: 84%
• Peptide Sequence: CSSPPSSEFMDVN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 120kDa band observed in Human Testis lysates and in lysates of cell line Jurkat (calculated MW of 110kDa according to NP_004642.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ideguchi et al, Biochem J. 2002 Oct 1;367(Pt 1):87-95.; PMID: 12084015). Recommended concentration: 1-3ug/ml.
• Reference: * Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y.; Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM.; Biochem J. 2002 Oct 1;367(Pt 1):87-95.; Pubmed 12084015

Target Info

• Gene Symbol: USP11
• Gene Full Name: ubiquitin specific peptidase 11
• Alias Symbols: deubiquitinating enzyme 11;ubiquitin carboxyl-terminal hydrolase 11;ubiquitin carboxyl-terminal hydrolase, X-linked;ubiquitin thioesterase 11;ubiquitin thiolesterase 11;ubiquitin-specific processing protease 11;UHX1.
• NCBI Gene Id: 8237
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 11
• Uniprot ID: P51784
• Protein Accession #: NP_004642.2
• Molecular Weight: 110